Publications

2021

Structural analysis of cross α-helical nanotubes provides insight into the designability of filamentous peptide nanomaterials. *Fengbin Wang*, Ordy Gnewou, Charles Modlin, *Leticia C Beltran*, Chunfu Xu, Zhangli Su, Puneet Juneja, Gevorg Grigoryan, *Edward H Egelman*, Vincent P Conticello Published in Nature communications, January 2021 (see publication) Research themes: Type: Paper

Cryoelectron-microscopy structure of the enteropathogenic *Weili Zheng*, Alejandro Peña, Aravindan Ilangovan, Jasmine Naemi-Baghshomali Clark, Gad Frankel, *Edward H Egelman*, Tiago R D Costa Published in Proceedings of the National Academy of Sciences of the United States of America, January 2021 (see publication) Research themes: Type: Paper, Corresponding author

2020

Cryoelectron-Microscopic Structure of the pKpQIL Conjugative Pili from Carbapenem-Resistant Klebsiella pneumoniae. *Weili Zheng*, Alejandro Pena, Wen Wen Low, Joshua L C Wong, Gad Frankel, *Edward H Egelman* Published in Structure (London, England : 1993), September 2020 (see publication) Research themes: Type: Paper, Corresponding author

Artificial Intracellular Filaments. Zhaoqianqi Feng, Huaimin Wang, *Fengbin Wang*, Younghoon Oh, Cristina Berciu, Qiang Cui, *Edward H Egelman*, Bing Xu Published in Cell reports. Physical science, August 2020 (see publication) Research themes: Type: Paper

Structures of filamentous viruses infecting hyperthermophilic archaea explain DNA stabilization in extreme environments. *Fengbin Wang*, Diana P Baquero, *Leticia C Beltran*, Zhangli Su, *Tomasz Osinski*, *Weili Zheng*, David Prangishvili, Mart Krupovic, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, August 2020 (see publication) Research themes: Type: Paper, Corresponding author

The structures of two archaeal type IV pili illuminate evolutionary relationships. *Fengbin Wang*, Diana P Baquero, Zhangli Su, *Leticia C Beltran*, David Prangishvili, Mart Krupovic, *Edward H Egelman* Published in Nature communications, July 2020 (see publication) Research themes: Type: Paper, Corresponding author

Atomic structure of the Campylobacter jejuni flagellar filament reveals how ε Proteobacteria escaped Toll-like receptor 5 surveillance *Mark A B Kreutzberger*, Cheryl Ewing, Frederic Poly, *Fengbin Wang*, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, July 2020 (see publication) Research themes: Type: Paper, Corresponding author

Osteogenesis imperfecta mutations in plastin 3 lead to impaired calcium regulation of actin bundling. Christopher L Schwebach, Elena Kudryashova, *Weili Zheng*, Matthew Orchard, Harper Smith, Lucas A Runyan, *Edward H Egelman*, Dmitri S Kudryashov Published in Bone research, June 2020 (see publication) Research themes: Type: Paper

Structure of a filamentous virus uncovers familial ties within the archaeal virosphere. *Fengbin Wang*, Diana P Baquero, Zhangli Su, *Tomasz Osinski*, David Prangishvili, *Edward H Egelman*, Mart Krupovic Published in Virus evolution, May 2020 (see publication) Research themes: Type: Paper

Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires. Yun X Chen, Nicole L Ing, *Fengbin Wang*, Dawei Xu, Nancy B Sloan, Nga T Lam, Daniel L Winter, *Edward H Egelman*, Allon I Hochbaum, Douglas S Clark, Dominic J Glover Published in ACS nano, April 2020 (see publication) Research themes: Type: Paper

The structure of helical lipoprotein lipase reveals an unexpected twist in lipase storage. Kathryn H Gunn, Benjamin S Roberts, *Fengbin Wang*, Joshua D Strauss, Mario J Borgnia, *Edward H Egelman*, Saskia B Neher Published in Proceedings of the National Academy of Sciences of the United States of America, April 2020 (see publication) Research themes: Type: Paper

Cryo-EM: Ice Is Nice, but Good Ice Can Be Hard to Find. *Edward H Egelman* Published in Biophysical journal, February 2020 (see publication) Research themes: Type: Paper, Corresponding author

2019

A packing for A-form DNA in an icosahedral virus. *Fengbin Wang*, Ying Liu, Zhangli Su, *Tomasz Osinski*, Guilherme A P de Oliveira, James F Conway, Stefan Schouten, Mart Krupovic, David Prangishvili, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, October 2019 (see publication) Research themes: Type: Paper, Corresponding author

Hooked on motility. *Edward H Egelman* Published in Nature structural & molecular biology, October 2019 (see publication) Research themes: Type: Paper, Corresponding author

The AAA + ATPase TorsinA polymerizes into hollow helical tubes with 8.5 subunits per turn. F Esra Demircioglu, *Weili Zheng*, Alexander J McQuown, Nolan K Maier, Nicki Watson, Iain M Cheeseman, Vladimir Denic, *Edward H Egelman*, Thomas U Schwartz Published in Nature communications, July 2019 (see publication) Research themes: Type: Paper

Ambidextrous helical nanotubes from self-assembly of designed helical hairpin motifs. Spencer A Hughes, *Fengbin Wang*, Shengyuan Wang, *Mark A B Kreutzberger*, *Tomasz Osinski*, Albina Orlova, Joseph S Wall, Xiaobing Zuo, *Edward H Egelman*, Vincent P Conticello Published in Proceedings of the National Academy of Sciences of the United States of America, July 2019 (see publication) Research themes: Type: Paper

An extensively glycosylated archaeal pilus survives extreme conditions. *Fengbin Wang*, Virginija Cvirkaite-Krupovic, *Mark A B Kreutzberger*, Zhangli Su, Guilherme A P de Oliveira, *Tomasz Osinski*, Nicholas Sherman, Frank DiMaio, Joseph S Wall, David Prangishvili, Mart Krupovic, *Edward H Egelman* Published in Nature microbiology, May 2019 (see publication) Research themes: Type: Paper, Corresponding author

Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus. Benjamin Bardiaux, Gisele Cardoso de Amorim, Areli Luna Rico, *Weili Zheng*, Ingrid Guilvout, Camille Jollivet, Michael Nilges, *Edward H Egelman*, Nadia Izadi-Pruneyre, Olivera Francetic Published in Structure (London, England : 1993), May 2019 (see publication) Research themes: Type: Paper

Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers. *Fengbin Wang*, Yangqi Gu, J Patrick O'Brien, Sophia M Yi, Sibel Ebru Yalcin, Vishok Srikanth, Cong Shen, Dennis Vu, Nicole L Ing, Allon I Hochbaum, *Edward H Egelman*, Nikhil S Malvankar Published in Cell, April 2019 (see publication) Research themes: Type: Paper, Corresponding author

Molecular mechanism for NLRP6 inflammasome assembly and activation. Chen Shen, Alvin Lu, Wen Jun Xie, Jianbin Ruan, Roberto Negro, *Edward H Egelman*, Tian-Min Fu, Hao Wu Published in Proceedings of the National Academy of Sciences of the United States of America, January 2019 (see publication) Research themes: Type: Paper

2018

Functional reconstitution of the type IVa pilus assembly system from enterohaemorrhagic Escherichia coli. Areli Luna Rico, *Weili Zheng*, Nathalie Petiot, *Edward H Egelman*, Olivera Francetic Published in Molecular microbiology, December 2018 (see publication) Research themes: Type: Paper

Cryo-EM structure of the NLRC4 Mariusz Matyszewski, *Weili Zheng*, Jacob Lueck, Brendan Antiochos, *Edward H Egelman*, Jungsan Sohn Published in The Journal of biological chemistry, November 2018 (see publication) Research themes: Type: Paper, Corresponding author

Structural conservation in a membrane-enveloped filamentous virus infecting a hyperthermophilic acidophile. Ying Liu, *Tomasz Osinski*, *Fengbin Wang*, Mart Krupovic, Stefan Schouten, Peter Kasson, David Prangishvili, *Edward H Egelman* Published in Nature communications, August 2018 (see publication) Research themes: Type: Paper, Corresponding author

Functional role of the type 1 pilus rod structure in mediating host-pathogen interactions. Caitlin N Spaulding, Henry Louis Schreiber, *Weili Zheng*, Karen W Dodson, Jennie E Hazen, Matt S Conover, *Fengbin Wang*, Pontus Svenmarker, Areli Luna-Rico, Olivera Francetic, Magnus Andersson, Scott Hultgren, *Edward H Egelman* Published in eLife, January 2018 (see publication) Research themes: Type: Paper

2017

Structural basis for high-affinity actin binding revealed by a β-III-spectrin SCA5 missense mutation. Adam W Avery, Michael E Fealey, *Fengbin Wang*, Albina Orlova, Andrew R Thompson, David D Thomas, Thomas S Hays, *Edward H Egelman* Published in Nature communications, November 2017 (see publication) Research themes: Type: Paper

A structural model of flagellar filament switching across multiple bacterial species. *Fengbin Wang*, Andrew M Burrage, Sandra Postel, Reece E Clark, Albina Orlova, Eric J Sundberg, Daniel B Kearns, *Edward H Egelman* Published in Nature communications, October 2017 (see publication) Research themes: Type: Paper, Corresponding author

Structure of the calcium-dependent type 2 secretion pseudopilus. Aracelys López-Castilla, Jenny-Lee Thomassin, Benjamin Bardiaux, *Weili Zheng*, Mangayarkarasi Nivaskumar, Xiong Yu, Michael Nilges, *Edward H Egelman*, Nadia Izadi-Pruneyre, Olivera Francetic Published in Nature microbiology, October 2017 (see publication) Research themes: Type: Paper

Cryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution. *Fengbin Wang*, Mathieu Coureuil, *Tomasz Osinski*, Albina Orlova, Tuba Altindal, Gaël Gesbert, Xavier Nassif, *Edward H Egelman*, Lisa Craig Published in Structure (London, England : 1993), September 2017 (see publication) Research themes: Type: Paper, Corresponding author

Refined Cryo-EM Structure of the T4 Tail Tube: Exploring the Lowest Dose Limit. *Weili Zheng*, *Fengbin Wang*, Nicholas M I Taylor, Ricardo C Guerrero-Ferreira, Petr G Leiman, *Edward H Egelman* Published in Structure (London, England : 1993), August 2017 (see publication) Research themes: Type: Paper, Corresponding author

Structural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signaling. Thomas Ve, Parimala R Vajjhala, Andrew Hedger, Tristan Croll, Frank DiMaio, Shane Horsefield, Xiong Yu, Peter Lavrencic, Zahid Hassan, Garry P Morgan, Ashley Mansell, Mehdi Mobli, Ailis O'Carroll, Brieuc Chauvin, Yann Gambin, Emma Sierecki, Michael J Landsberg, Katryn J Stacey, *Edward H Egelman*, Bostjan Kobe Published in Nature structural & molecular biology, August 2017 (see publication) Research themes: Type: Paper

Model for a novel membrane envelope in a filamentous hyperthermophilic virus. Peter Kasson, Frank DiMaio, Xiong Yu, Soizick Lucas-Staat, Mart Krupovic, Stefan Schouten, David Prangishvili, *Edward H Egelman* Published in eLife, June 2017 (see publication) Research themes: Type: Paper

RosettaES: a sampling strategy enabling automated interpretation of difficult cryo-EM maps. Brandon Frenz, Alexandra C Walls, *Edward H Egelman*, David Veesler, Frank DiMaio Published in Nature methods, June 2017 (see publication) Research themes: Type: Paper

Cryo-EM of bacterial pili and archaeal flagellar filaments. *Edward H Egelman* Published in Current opinion in structural biology, June 2017 (see publication) Research themes: Type: Paper, Corresponding author

2016

Cryo-EM Structure of Caspase-8 Tandem DED Filament Reveals Assembly and Regulation Mechanisms of the Death-Inducing Signaling Complex. Tian-Min Fu, Yang Li, Alvin Lu, Zongli Li, Parimala R Vajjhala, Anthony C Cruz, Devendra B Srivastava, Frank DiMaio, Pawel A Penczek, Richard M Siegel, Katryn J Stacey, *Edward H Egelman*, Hao Wu Published in Molecular cell, October 2016 (see publication) Research themes: Type: Paper

Bacterial flagellar capping proteins adopt diverse oligomeric states. Sandra Postel, Daniel Deredge, Daniel A Bonsor, Xiong Yu, Kay Diederichs, Saskia Helmsing, Aviv Vromen, Assaf Friedler, Michael Hust, *Edward H Egelman*, Dorothy Beckett, Patrick L Wintrode, Eric J Sundberg Published in eLife, October 2016 (see publication) Research themes: Type: Paper

Structure of the Neisseria meningitidis Type IV pilus. Subramania Kolappan, Mathieu Coureuil, Xiong Yu, Xavier Nassif, *Edward H Egelman*, Lisa Craig Published in Nature communications, October 2016 (see publication) Research themes: Type: Paper

Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex. Tiago R D Costa, Aravindan Ilangovan, Marta Ukleja, Adam Redzej, Joanne M Santini, Terry K Smith, *Edward H Egelman*, Gabriel Waksman Published in Cell, September 2016 (see publication) Research themes: Type: Paper, Corresponding author

Archaeal flagellin combines a bacterial type IV pilin domain with an Ig-like domain. Tatjana Braun, Matthijn R Vos, Nir Kalisman, Nicholas E Sherman, Reinhard Rachel, Reinhard Wirth, Gunnar F Schröder, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, September 2016 (see publication) Research themes: Type: Paper, Corresponding author

Resolution advances in cryo-EM enable application to drug discovery. Sriram Subramaniam, Lesley A Earl, Veronica Falconieri, Jacqueline Ls Milne, *Edward H Egelman* Published in Current opinion in structural biology, August 2016 (see publication) Research themes: Type: Paper

The Current Revolution in Cryo-EM. *Edward H Egelman* Published in Biophysical journal, March 2016 (see publication) Research themes: Type: Paper, Corresponding author

Biophysical Journal Special Issue: Focus on Cryo-EM. *Edward H Egelman*, Andreas Engel Published in Biophysical journal, February 2016 (see publication) Research themes: Type: Paper, Corresponding author

Structure of a Chaperone-Usher Pilus Reveals the Molecular Basis of Rod Uncoiling. Manuela K Hospenthal, Adam Redzej, Karen Dodson, Marta Ukleja, Brandon Frenz, Catarina Rodrigues, Scott J Hultgren, Frank DiMaio, *Edward H Egelman*, Gabriel Waksman Published in Cell, January 2016 (see publication) Research themes: Type: Paper, Corresponding author

2015

Actin polymerization is stimulated by actin cross-linking protein palladin. Ritu Gurung, Rahul Yadav, Joseph G Brungardt, Albina Orlova, *Edward H Egelman*, Moriah R Beck Published in The Biochemical journal, November 2015 (see publication) Research themes: Type: Paper

Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy. Lorenzo Sborgi, Francesco Ravotti, Venkata P Dandey, Mathias S Dick, Adam Mazur, Sina Reckel, Mohamed Chami, Sebastian Scherer, Matthias Huber, Anja Böckmann, *Edward H Egelman*, Henning Stahlberg, Petr Broz, Beat H Meier, Sebastian Hiller Published in Proceedings of the National Academy of Sciences of the United States of America, October 2015 (see publication) Research themes: Type: Paper

Assembly-driven activation of the AIM2 foreign-dsDNA sensor provides a polymerization template for downstream ASC. Seamus R Morrone, Mariusz Matyszewski, Xiong Yu, Michael Delannoy, *Edward H Egelman*, Jungsan Sohn Published in Nature communications, July 2015 (see publication) Research themes: Type: Paper

Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination. Martin R G Taylor, Mário Špírek, Kathy R Chaurasiya, Jordan D Ward, Raffaella Carzaniga, Xiong Yu, *Edward H Egelman*, Lucy M Collinson, David Rueda, Lumir Krejci, Simon J Boulton Published in Cell, July 2015 (see publication) Research themes: Type: Paper

The molecular basis for flexibility in the flexible filamentous plant viruses. Frank DiMaio, Chun-Chieh Chen, Xiong Yu, Brandon Frenz, Yau-Heiu Hsu, Na-Sheng Lin, *Edward H Egelman* Published in Nature structural & molecular biology, July 2015 (see publication) Research themes: Type: Paper

Archaeal actin from a hyperthermophile forms a single-stranded filament. Tatjana Braun, Albina Orlova, Karin Valegård, Ann-Christin Lindås, Gunnar F Schröder, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, July 2015 (see publication) Research themes: Type: Paper, Corresponding author

Virology. A virus that infects a hyperthermophile encapsidates A-form DNA. Frank DiMaio, Xiong Yu, Elena Rensen, Mart Krupovic, David Prangishvili, *Edward H Egelman* Published in Science (New York, N.Y.), May 2015 (see publication) Research themes: Type: Paper, Corresponding author

Three-dimensional reconstruction of helical polymers. *Edward H Egelman* Published in Archives of biochemistry and biophysics, April 2015 (see publication) Research themes: Type: Paper, Corresponding author

Reproducibility of research in biophysics. Les Loew, Dorothy Beckett, *Edward H Egelman*, Suzanne Scarlata Published in Biophysical journal, April 2015 (see publication) Research themes: Type: Paper

Structure of the type VI secretion system contractile sheath. Mikhail Kudryashev, Ray Yu-Ruei Wang, Maximilian Brackmann, Sebastian Scherer, Timm Maier, David Baker, Frank DiMaio, Henning Stahlberg, *Edward H Egelman*, Marek Basler Published in Cell, February 2015 (see publication) Research themes: Type: Paper, Corresponding author

De novo protein structure determination from near-atomic-resolution cryo-EM maps. Ray Yu-Ruei Wang, Mikhail Kudryashev, Xueming Li, *Edward H Egelman*, Marek Basler, Yifan Cheng, David Baker, Frank DiMaio Published in Nature methods, February 2015 (see publication) Research themes: Type: Paper

Structural plasticity of helical nanotubes based on coiled-coil assemblies. *E H Egelman*, C Xu, F DiMaio, E Magnotti, C Modlin, X Yu, E Wright, D Baker, V P Conticello Published in Structure (London, England : 1993), January 2015 (see publication) Research themes: Type: Paper, Corresponding author

2014

Near-atomic resolution for one state of F-actin. Vitold E Galkin, Albina Orlova, Matthijn R Vos, Gunnar F Schröder, *Edward H Egelman* Published in Structure (London, England : 1993), December 2014 (see publication) Research themes: Type: Paper, Corresponding author

Ambiguities in helical reconstruction. *Edward H Egelman* Published in eLife, December 2014 (see publication) Research themes: Type: Paper

A PH domain in ACAP1 possesses key features of the BAR domain in promoting membrane curvature. Xiaoyun Pang, Jun Fan, Yan Zhang, Kai Zhang, Bingquan Gao, Jun Ma, Jian Li, Yuchen Deng, Qiangjun Zhou, *Edward H Egelman*, Victor W Hsu, Fei Sun Published in Developmental cell, October 2014 (see publication) Research themes: Type: Paper

Direct interaction of actin filaments with F-BAR protein pacsin2. Julius Kostan, Ulrich Salzer, Albina Orlova, Imre Törö, Vesna Hodnik, Yosuke Senju, Juan Zou, Claudia Schreiner, Julia Steiner, Jari Meriläinen, Marko Nikki, Ismo Virtanen, Oliviero Carugo, Juri Rappsilber, Pekka Lappalainen, Veli-Pekka Lehto, Gregor Anderluh, *Edward H Egelman*, Kristina Djinović-Carugo Published in EMBO reports, September 2014 (see publication) Research themes: Type: Paper

Molecular imprinting as a signal-activation mechanism of the viral RNA sensor RIG-I. Bin Wu, Alys Peisley, David Tetrault, Zongli Li, *Edward H Egelman*, Katharine E Magor, Thomas Walz, Pawel A Penczek, Sun Hur Published in Molecular cell, July 2014 (see publication) Research themes: Type: Paper

Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties. Peter M Thompson, Caitlin E Tolbert, Kai Shen, Pradeep Kota, Sean M Palmer, Karen M Plevock, Albina Orlova, Vitold E Galkin, Keith Burridge, *Edward H Egelman*, Nikolay V Dokholyan, Richard Superfine, Sharon L Campbell Published in Structure (London, England : 1993), April 2014 (see publication) Research themes: Type: Paper

Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers. Mangayarkarasi Nivaskumar, Guillaume Bouvier, Manuel Campos, Nathalie Nadeau, Xiong Yu, *Edward H Egelman*, Michael Nilges, Olivera Francetic Published in Structure (London, England : 1993), April 2014 (see publication) Research themes: Type: Paper

Unified polymerization mechanism for the assembly of ASC-dependent inflammasomes. Alvin Lu, Venkat Giri Magupalli, Jianbin Ruan, Qian Yin, Maninjay K Atianand, Matthijn R Vos, Gunnar F Schröder, Katherine A Fitzgerald, Hao Wu, *Edward H Egelman* Published in Cell, March 2014 (see publication) Research themes: Type: Paper

2013

Structural architecture of the CARMA1/Bcl10/MALT1 signalosome: nucleation-induced filamentous assembly. Qi Qiao, Chenghua Yang, Chao Zheng, Lorena Fontán, Liron David, Xiong Yu, Clay Bracken, Monica Rosen, Ari Melnick, *Edward H Egelman*, Hao Wu Published in Molecular cell, October 2013 (see publication) Research themes: Type: Paper

Organization of F-actin by Fesselin (avian smooth muscle synaptopodin 2). Mechthild M Schroeter, Albina Orlova, *Edward H Egelman*, Brent Beall, Joseph M Chalovich Published in Biochemistry, June 2013 (see publication) Research themes: Type: Paper

2012

The structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism. Vitold E Galkin, Subramaniapillai Kolappan, Dixon Ng, ZuSheng Zong, Juliana Li, Xiong Yu, *Edward H Egelman*, Lisa Craig Published in Journal of bacteriology, November 2012 (see publication) Research themes: Type: Paper

MDA5 assembles into a polar helical filament on dsRNA. Ian C Berke, Xiong Yu, Yorgo Modis, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, October 2012 (see publication) Research themes: Type: Paper

Are ParM filaments polar or bipolar? Vitold E Galkin, Albina Orlova, *Edward H Egelman* Published in Journal of molecular biology, August 2012 (see publication) Research themes: Type: Paper

Filaments from Ignicoccus hospitalis show diversity of packing in proteins containing N-terminal type IV pilin helices. Xiong Yu, Charles Goforth, Carolin Meyer, Reinhard Rachel, Reinhard Wirth, Gunnar F Schröder, *Edward H Egelman* Published in Journal of molecular biology, June 2012 (see publication) Research themes: Type: Paper

Stepwise molecular display utilizing icosahedral and helical complexes of phage coat and decoration proteins in the development of robust nanoscale display vehicles. Kristin N Parent, Christina T Deedas, *Edward H Egelman*, Sherwood R Casjens, Timothy S Baker, Carolyn M Teschke Published in Biomaterials, May 2012 (see publication) Research themes: Type: Paper

Structure of the Vibrio cholerae Type IVb Pilus and stability comparison with the Neisseria gonorrhoeae type IVa pilus. Juliana Li, *Edward H Egelman*, Lisa Craig Published in Journal of molecular biology, March 2012 (see publication) Research themes: Type: Paper

Outcome of the first electron microscopy validation task force meeting. Richard Henderson, Andrej Sali, Matthew L Baker, Bridget Carragher, Batsal Devkota, Kenneth H Downing, *Edward H Egelman*, Zukang Feng, Joachim Frank, Nikolaus Grigorieff, Wen Jiang, Steven J Ludtke, Ohad Medalia, Pawel A Penczek, Peter B Rosenthal, Michael G Rossmann, Michael F Schmid, Gunnar F Schröder, Alasdair C Steven, David L Stokes, John D Westbrook, Willy Wriggers, Huanwang Yang, Jasmine Young, Helen M Berman, Wah Chiu, Gerard J Kleywegt, Catherine L Lawson Published in Structure (London, England : 1993), February 2012 (see publication) Research themes: Type: Paper

Actin filaments as tension sensors. Vitold E Galkin, Albina Orlova, *Edward H Egelman* Published in Current biology : CB, February 2012 (see publication) Research themes: Type: Paper

Real-space processing of helical filaments in SPARX. Elmar Behrmann, Guozhi Tao, David L Stokes, *Edward H Egelman*, Stefan Raunser, Pawel A Penczek Published in Journal of structural biology, January 2012 (see publication) Research themes: Type: Paper

2011

Remodeling of actin filaments by ADF/cofilin proteins. Vitold E Galkin, Albina Orlova, Dmitri S Kudryashov, Alexander Solodukhin, Emil Reisler, Gunnar F Schröder, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, December 2011 (see publication) Research themes: Type: Paper

The N-terminal domains of myosin binding protein C can bind polymorphically to F-actin. Albina Orlova, Vitold E Galkin, Cy M J Jeffries, *Edward H Egelman*, Jill Trewhella Published in Journal of molecular biology, August 2011 (see publication) Research themes: Type: Paper

A257T linker region mutant of T7 helicase-primase protein is defective in DNA loading and rescued by T7 DNA polymerase. Gayatri Patel, Daniel S Johnson, Bo Sun, Manjula Pandey, Xiong Yu, *Edward H Egelman*, Michelle D Wang, Smita S Patel Published in The Journal of biological chemistry, April 2011 (see publication) Research themes: Type: Paper

Two modes of binding of DinI to RecA filament provide a new insight into the regulation of SOS response by DinI protein. Vitold E Galkin, Rachel L Britt, Lukas B Bane, Xiong Yu, Michael M Cox, *Edward H Egelman* Published in Journal of molecular biology, April 2011 (see publication) Research themes: Type: Paper

2010

Cryo-reconstructions of P22 polyheads suggest that phage assembly is nucleated by trimeric interactions among coat proteins. Kristin N Parent, Robert S Sinkovits, Margaret M Suhanovsky, Carolyn M Teschke, *Edward H Egelman*, Timothy S Baker Published in Physical biology, December 2010 (see publication) Research themes: Type: Paper

Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW. Stefan Gajewski, Michael R Webb, Vitold Galkin, *Edward H Egelman*, Kenneth N Kreuzer, Stephen W White Published in Journal of molecular biology, November 2010 (see publication) Research themes: Type: Paper

Structural polymorphism in F-actin. Vitold E Galkin, Albina Orlova, Gunnar F Schröder, *Edward H Egelman* Published in Nature structural & molecular biology, October 2010 (see publication) Research themes: Type: Paper

Reconstruction of helical filaments and tubes. *Edward H Egelman* Published in Methods in enzymology, October 2010 (see publication) Research themes: Type: Paper

Helical filaments of human Dmc1 protein on single-stranded DNA: a cautionary tale. Xiong Yu, *Edward H Egelman* Published in Journal of molecular biology, July 2010 (see publication) Research themes: Type: Paper

Opening of tandem calponin homology domains regulates their affinity for F-actin. Vitold E Galkin, Albina Orlova, Anita Salmazo, Kristina Djinovic-Carugo, *Edward H Egelman* Published in Nature structural & molecular biology, April 2010 (see publication) Research themes: Type: Paper

Mapping of drebrin binding site on F-actin. Elena E Grintsevich, Vitold E Galkin, Albina Orlova, A Jimmy Ytterberg, Mouna M Mikati, Dmitri S Kudryashov, Joseph A Loo, *Edward H Egelman*, Emil Reisler Published in Journal of molecular biology, March 2010 (see publication) Research themes: Type: Paper

Structure and function of large cellular assemblies. Nenad Ban, *Edward H Egelman* Published in Current opinion in structural biology, March 2010 (see publication) Research themes: Type: Paper

ParA2, a Vibrio cholerae chromosome partitioning protein, forms left-handed helical filaments on DNA. Monica P Hui, Vitold E Galkin, Xiong Yu, Alicja Z Stasiak, Andrzej Stasiak, Matthew K Waldor, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, February 2010 (see publication) Research themes: Type: Paper

The structure of the Salmonella typhimurium type III secretion system needle shows divergence from the flagellar system. Vitold E Galkin, Wolfgang H Schmied, Oliver Schraidt, Thomas C Marlovits, *Edward H Egelman* Published in Journal of molecular biology, January 2010 (see publication) Research themes: Type: Paper

2009

Reducing irreducible complexity: divergence of quaternary structure and function in macromolecular assemblies. *Edward H Egelman* Published in Current opinion in cell biology, December 2009 (see publication) Research themes: Type: Paper, Corresponding author

Divergence of the flagellar hook and filament. *Edward H Egelman* Published in Structure (London, England : 1993), November 2009 (see publication) Research themes: Type: Paper

Structural polymorphism of the ParM filament and dynamic instability. Vitold E Galkin, Albina Orlova, Chris Rivera, R Dyche Mullins, *Edward H Egelman* Published in Structure (London, England : 1993), September 2009 (see publication) Research themes: Type: Paper

Electron microscopy of helical filaments: rediscovering buried treasures in negative stain. *Edward H Egelman*, Linda A Amos Published in BioEssays : news and reviews in molecular, cellular and developmental biology, July 2009 (see publication) Research themes: Type: Paper, Corresponding author

The RecB nuclease domain binds to RecA-DNA filaments: implications for filament loading. Debora Lucarelli, Ying A Wang, Vitold E Galkin, Xiong Yu, Dale B Wigley, *Edward H Egelman* Published in Journal of molecular biology, June 2009 (see publication) Research themes: Type: Paper

Problems in fitting high resolution structures into electron microscopic reconstructions. *Edward H Egelman* Published in HFSP journal, May 2009 (see publication) Research themes: Type: Paper

The bipolar filaments formed by herpes simplex virus type 1 SSB/recombination protein (ICP8) suggest a mechanism for DNA annealing. Alexander M Makhov, Anindito Sen, Xiong Yu, Martha N Simon, Jack D Griffith, *Edward H Egelman* Published in Journal of molecular biology, January 2009 (see publication) Research themes: Type: Paper

Implications of the RecA structure. *Edward H Egelman* Published in F1000 biology reports, January 2009 (see publication) Research themes: Type: Paper, Corresponding author

2008

Loop 2 in Saccharomyces cerevisiae Rad51 protein regulates filament formation and ATPase activity. Xiao-Ping Zhang, Vitold E Galkin, Xiong Yu, *Edward H Egelman*, Wolf-Dietrich Heyer Published in Nucleic acids research, November 2008 (see publication) Research themes: Type: Paper

Cleavage of bacteriophage lambda cI repressor involves the RecA C-terminal domain. Vitold E Galkin, Xiong Yu, Jakub Bielnicki, Dieudonné Ndjonka, Charles E Bell, *Edward H Egelman* Published in Journal of molecular biology, November 2008 (see publication) Research themes: Type: Paper

The structure of F-pili. Ying A Wang, Xiong Yu, Philip M Silverman, Robin L Harris, *Edward H Egelman* Published in Journal of molecular biology, November 2008 (see publication) Research themes: Type: Paper

Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24. Sara Ghazi-Tabatabai, Suraj Saksena, Judith M Short, Ajaybabu V Pobbati, Dmitry B Veprintsev, R Anthony Crowther, Scott D Emr, *Edward H Egelman*, Roger L Williams Published in Structure (London, England : 1993), September 2008 (see publication) Research themes: Type: Paper

Functional and structural basis for a bacteriophage homolog of human RAD52. Mickaël Ploquin, Ali Bransi, Eric R Paquet, Alicja Z Stasiak, Andrzej Stasiak, Xiong Yu, Anna M Cieslinska, *Edward H Egelman*, Sylvain Moineau, Jean-Yves Masson Published in Current biology : CB, July 2008 (see publication) Research themes: Type: Paper

Helicity in electron microscopy images-a comment on Wang TF, Chen LT and Wang AH 2008 BioEssays 30:48-56. *Edward H Egelman* Published in BioEssays : news and reviews in molecular, cellular and developmental biology, July 2008 (see publication) Research themes: Type: Paper

The structure of an archaeal pilus. Ying A Wang, Xiong Yu, Sandy Y M Ng, Ken F Jarrell, *Edward H Egelman* Published in Journal of molecular biology, July 2008 (see publication) Research themes: Type: Paper

A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments. Sean D Sheridan, Xiong Yu, Robyn Roth, John E Heuser, Michael G Sehorn, Patrick Sung, *Edward H Egelman*, Douglas K Bishop Published in Nucleic acids research, June 2008 (see publication) Research themes: Type: Paper

Divergence of quaternary structures among bacterial flagellar filaments. Vitold E Galkin, Xiong Yu, Jakub Bielnicki, John Heuser, Cheryl P Ewing, Patricia Guerry, *Edward H Egelman* Published in Science (New York, N.Y.), April 2008 (see publication) Research themes: Type: Paper

Macromolecular assemblages. Editorial overview. *Edward H Egelman*, Andrew Gw Leslie Published in Current opinion in structural biology, April 2008 (see publication) Research themes: Type: Paper

Structural basis of membrane invagination by F-BAR domains. Adam Frost, Rushika Perera, Aurélien Roux, Krasimir Spasov, Olivier Destaing, *Edward H Egelman*, Pietro De Camilli, Vinzenz M Unger Published in Cell, March 2008 (see publication) Research themes: Type: Paper

High-resolution cryo-EM structure of the F-actin-fimbrin/plastin ABD2 complex. Vitold E Galkin, Albina Orlova, Olga Cherepanova, Marie-Christine Lebart, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, February 2008 (see publication) Research themes: Type: Paper

Assembly of Weibel-Palade body-like tubules from N-terminal domains of von Willebrand factor. Ren-Huai Huang, Ying Wang, Robyn Roth, Xiong Yu, Angie R Purvis, John E Heuser, *Edward H Egelman*, J Evan Sadler Published in Proceedings of the National Academy of Sciences of the United States of America, January 2008 (see publication) Research themes: Type: Paper

Coronin-1A stabilizes F-actin by bridging adjacent actin protomers and stapling opposite strands of the actin filament. Vitold E Galkin, Albina Orlova, William Brieher, Hao Yuan Kueh, Timothy J Mitchison, *Edward H Egelman* Published in Journal of molecular biology, January 2008 (see publication) Research themes: Type: Paper

2007

Actin hydrophobic loop 262-274 and filament nucleation and elongation. Alexander Shvetsov, Vitold E Galkin, Albina Orlova, Martin Phillips, Sarah E Bergeron, Peter A Rubenstein, *Edward H Egelman*, Emil Reisler Published in Journal of molecular biology, November 2007 (see publication) Research themes: Type: Paper

Novel pro- and anti-recombination activities of the Bloom’s syndrome helicase. Dmitry V Bugreev, Xiong Yu, *Edward H Egelman*, Alexander V Mazin Published in Genes & development, November 2007 (see publication) Research themes: Type: Paper

Actin structure and function: what we still do not understand. Emil Reisler, *Edward H Egelman* Published in The Journal of biological chemistry, October 2007 (see publication) Research themes: Type: Paper

The structure of bacterial ParM filaments. Albina Orlova, Ethan C Garner, Vitold E Galkin, John Heuser, R Dyche Mullins, *Edward H Egelman* Published in Nature structural & molecular biology, September 2007 (see publication) Research themes: Type: Paper

Single-particle reconstruction from EM images of helical filaments. *Edward H Egelman* Published in Current opinion in structural biology, September 2007 (see publication) Research themes: Type: Paper, Corresponding author

Rad51 and Rad54 ATPase activities are both required to modulate Rad51-dsDNA filament dynamics. Xuan Li, Xiao-Ping Zhang, Jachen A Solinger, Konstantin Kiianitsa, Xiong Yu, *Edward H Egelman*, Wolf-Dietrich Heyer Published in Nucleic acids research, June 2007 (see publication) Research themes: Type: Paper

Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2. Fumiko Esashi, Vitold E Galkin, Xiong Yu, *Edward H Egelman*, Stephen C West Published in Nature structural & molecular biology, May 2007 (see publication) Research themes: Type: Paper

Stabilization of RAD-51-DNA filaments via an interaction domain in Caenorhabditis elegans BRCA2. Mark I R Petalcorin, Vitold E Galkin, Xiong Yu, *Edward H Egelman*, Simon J Boulton Published in Proceedings of the National Academy of Sciences of the United States of America, May 2007 (see publication) Research themes: Type: Paper

Mapping the interaction of cofilin with subdomain 2 on actin. Sabrina A Benchaar, Yongming Xie, Martin Phillips, Rachel R Ogorzalek Loo, Vitold E Galkin, Albina Orlova, Mario Thevis, Andras Muhlrad, Steven C Almo, Joseph A Loo, *Edward H Egelman*, Emil Reisler Published in Biochemistry, January 2007 (see publication) Research themes: Type: Paper

2006

RecA assembly, one molecule at a time. *Edward H Egelman* Published in Structure (London, England : 1993), November 2006 (see publication) Research themes: Type: Paper

Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions. Lisa Craig, Niels Volkmann, Andrew S Arvai, Michael E Pique, Mark Yeager, *Edward H Egelman*, John A Tainer Published in Molecular cell, September 2006 (see publication) Research themes: Type: Paper

The iterative helical real space reconstruction method: surmounting the problems posed by real polymers. *Edward H Egelman* Published in Journal of structural biology, August 2006 (see publication) Research themes: Type: Paper, Corresponding author

Structural polymorphism in bacterial EspA filaments revealed by cryo-EM and an improved approach to helical reconstruction. Ying A Wang, Xiong Yu, Calvin Yip, Natalie C Strynadka, *Edward H Egelman* Published in Structure (London, England : 1993), July 2006 (see publication) Research themes: Type: Paper

The structure of a filamentous bacteriophage. Ying A Wang, Xiong Yu, Stacy Overman, Masamichi Tsuboi, George J Thomas, *Edward H Egelman* Published in Journal of molecular biology, July 2006 (see publication) Research themes: Type: Paper

The Rad51/RadA N-terminal domain activates nucleoprotein filament ATPase activity. Vitold E Galkin, Yan Wu, Xiao-Ping Zhang, Xinguo Qian, Yujiong He, Xiong Yu, Wolf-Dietrich Heyer, Yu Luo, *Edward H Egelman* Published in Structure (London, England : 1993), June 2006 (see publication) Research themes: Type: Paper

The CH-domain of calponin does not determine the modes of calponin binding to F-actin. Vitold E Galkin, Albina Orlova, Abdellatif Fattoum, Michael P Walsh, *Edward H Egelman* Published in Journal of molecular biology, April 2006 (see publication) Research themes: Type: Paper

Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface. D S Kudryashov, V E Galkin, A Orlova, M Phan, *E H Egelman*, E Reisler Published in Journal of molecular biology, March 2006 (see publication) Research themes: Type: Paper

2005

Xin-repeats and nebulin-like repeats bind to F-actin in a similar manner. Olga Cherepanova, Albina Orlova, Vitold E Galkin, Peter F M van der Ven, Dieter O Fürst, Jian-Ping Jin, *Edward H Egelman* Published in Journal of molecular biology, December 2005 (see publication) Research themes: Type: Paper

Atomic model of a myosin filament in the relaxed state. John L Woodhead, Fa-Qing Zhao, Roger Craig, *Edward H Egelman*, Lorenzo Alamo, Raúl Padrón Published in Nature, August 2005 (see publication) Research themes: Type: Paper

BRCA2 BRC motifs bind RAD51-DNA filaments. Vitold E Galkin, Fumiko Esashi, Xiong Yu, Shixin Yang, Stephen C West, *Edward H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, June 2005 (see publication) Research themes: Type: Paper

Refining the structure of the Halobacterium salinarum flagellar filament using the iterative helical real space reconstruction method: insights into polymorphism. Shlomo Trachtenberg, Vitold E Galkin, *Edward H Egelman* Published in Journal of molecular biology, February 2005 (see publication) Research themes: Type: Paper

The Arg non-receptor tyrosine kinase modifies F-actin structure. Vitold E Galkin, Albina Orlova, Anthony J Koleske, *Edward H Egelman* Published in Journal of molecular biology, January 2005 (see publication) Research themes: Type: Paper

Structural polymorphism of Methanothermobacter thermautotrophicus MCM. Yen-Ju Chen, Xiong Yu, Rajesh Kasiviswanathan, Jae-Ho Shin, Zvi Kelman, *Edward H Egelman* Published in Journal of molecular biology, January 2005 (see publication) Research themes: Type: Paper

2004

Actin-destabilizing factors disrupt filaments by means of a time reversal of polymerization. Albina Orlova, Alexander Shvetsov, Vitold E Galkin, Dmitry S Kudryashov, Peter A Rubenstein, *Edward H Egelman*, Emil Reisler Published in Proceedings of the National Academy of Sciences of the United States of America, December 2004 (see publication) Research themes: Type: Paper

Acrosomal actin: twists and turns of a versatile filament. *Edward H Egelman* Published in Current biology : CB, November 2004 (see publication) Research themes: Type: Paper

More insights into structural plasticity of actin binding proteins. *Edward H Egelman* Published in Structure (London, England : 1993), July 2004 (see publication) Research themes: Type: Paper

The stalk region of dynamin drives the constriction of dynamin tubes. Yen-Ju Chen, Peijun Zhang, *Edward H Egelman*, Jenny E Hinshaw Published in Nature structural & molecular biology, May 2004 (see publication) Research themes: Type: Paper

What is the structure of the RecA-DNA filament? X Yu, M S VanLoock, S Yang, J T Reese, *E H Egelman* Published in Current protein & peptide science, April 2004 (see publication) Research themes: Type: Paper

2003

ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments. Vitold E Galkin, Albina Orlova, Margaret S VanLoock, Alexander Shvetsov, Emil Reisler, *Edward H Egelman* Published in The Journal of cell biology, December 2003 (see publication) Research themes: Type: Paper

Issues of resolution and polymorphism in single-particle reconstruction. Shixin Yang, Xiong Yu, Vitold E Galkin, *Edward H Egelman* Published in Journal of structural biology, December 2003 (see publication) Research themes: Type: Paper

A DNA pairing-enhanced conformation of bacterial RecA proteins. Nami Haruta, Xiong Yu, Shixin Yang, *Edward H Egelman*, Michael M Cox Published in The Journal of biological chemistry, October 2003 (see publication) Research themes: Type: Paper

Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments. Margaret S VanLoock, Xiong Yu, Shixin Yang, Vitold E Galkin, Hao Huang, Shyamala S Rajan, Wayne F Anderson, Elizabeth A Stohl, H Steven Seifert, *Edward H Egelman* Published in Journal of molecular biology, October 2003 (see publication) Research themes: Type: Paper

Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms. Mirjana Lilic, Vitold E Galkin, Albina Orlova, Margaret S VanLoock, *Edward H Egelman*, C Erec Stebbins Published in Science (New York, N.Y.), September 2003 (see publication) Research themes: Type: Paper

Do the utrophin tandem calponin homology domains bind F-actin in a compact or extended conformation? Vitold E Galkin, Albina Orlova, Margaret S VanLoock, *Edward H Egelman* Published in Journal of molecular biology, August 2003 (see publication) Research themes: Type: Paper

A tale of two polymers: new insights into helical filaments. *Edward H Egelman* Published in Nature reviews. Molecular cell biology, August 2003 (see publication) Research themes: Type: Paper, Corresponding author

Cell walls, cell shape, and bacterial actin homologs. *Edward H Egelman* Published in Developmental cell, July 2003 (see publication) Research themes: Type: Paper

Actin’s prokaryotic homologs. *Edward H Egelman* Published in Current opinion in structural biology, May 2003 (see publication) Research themes: Type: Paper, Corresponding author

ATP-mediated conformational changes in the RecA filament. Margaret S VanLoock, Xiong Yu, Shixin Yang, Alex L Lai, Claudia Low, Michael J Campbell, *Edward H Egelman* Published in Structure (London, England : 1993), February 2003 (see publication) Research themes: Type: Paper

Helical structure of the needle of the type III secretion system of Shigella flexneri. Frank S Cordes, Kaoru Komoriya, Eric Larquet, Shixin Yang, *Edward H Egelman*, Ariel Blocker, Susan M Lea Published in The Journal of biological chemistry, February 2003 (see publication) Research themes: Type: Paper

The location of ubiquitin in Lethocerus arthrin. Vitold E Galkin, Albina Orlova, Natalya Lukoyanova, Margaret S VanLoock, Petra Hååg, Belinda Bullard, *Edward H Egelman* Published in Journal of molecular biology, January 2003 (see publication) Research themes: Type: Paper

2002

Flexibility of the rings: structural asymmetry in the DnaB hexameric helicase. Shixin Yang, Xiong Yu, Margaret S VanLoock, Maria J Jezewska, Wlodzimierz Bujalowski, *Edward H Egelman* Published in Journal of molecular biology, September 2002 (see publication) Research themes: Type: Paper

Structure and function of Hib pili from Haemophilus influenzae type b. Xiang-Qi Mu, *Edward H Egelman*, Esther Bullitt Published in Journal of bacteriology, August 2002 (see publication) Research themes: Type: Paper

How does ATP hydrolysis control actin’s associations? Elena P Sablin, John F Dawson, Margaret S VanLoock, James A Spudich, *Edward H Egelman*, Robert J Fletterick Published in Proceedings of the National Academy of Sciences of the United States of America, August 2002 (see publication) Research themes: Type: Paper

The Methanobacterium thermoautotrophicum MCM protein can form heptameric rings. Xiong Yu, Margaret S VanLoock, Andrzej Poplawski, Zvi Kelman, Tao Xiang, Bik K Tye, *Edward H Egelman* Published in EMBO reports, August 2002 (see publication) Research themes: Type: Paper

Homologous-pairing activity of the Bacillus subtilis bacteriophage SPP1 replication protein G35P. Silvia Ayora, Riccardo Missich, Pablo Mesa, Rudi Lurz, Shixin Yang, *Edward H Egelman*, Juan C Alonso Published in The Journal of biological chemistry, July 2002 (see publication) Research themes: Type: Paper

The bacterial protein SipA polymerizes G-actin and mimics muscle nebulin. Vitold E Galkin, Albina Orlova, Margaret S VanLoock, Daoguo Zhou, Jorge E Galán, *Edward H Egelman* Published in Nature structural biology, June 2002 (see publication) Research themes: Type: Paper

The hexameric ring structure of the Escherichia coli RuvB branch migration protein. Yen-Ju Chen, Xiong Yu, *Edward H Egelman* Published in Journal of molecular biology, June 2002 (see publication) Research themes: Type: Paper

The utrophin actin-binding domain binds F-actin in two different modes: implications for the spectrin superfamily of proteins. Vitold E Galkin, Albina Orlova, Margaret S VanLoock, Inna N Rybakova, James M Ervasti, *Edward H Egelman* Published in The Journal of cell biology, April 2002 (see publication) Research themes: Type: Paper

A new internal mode in F-actin helps explain the remarkable evolutionary conservation of actin’s sequence and structure. Vitold E Galkin, Margaret S VanLoock, Albina Orlova, *Edward H Egelman* Published in Current biology : CB, April 2002 (see publication) Research themes: Type: Paper

SV40 large T antigen hexamer structure: domain organization and DNA-induced conformational changes. Margaret S VanLoock, Alexander Alexandrov, Xiong Yu, Nicholas R Cozzarelli, *Edward H Egelman* Published in Current biology : CB, March 2002 (see publication) Research themes: Type: Paper

Each actin subunit has three nebulin binding sites: implications for steric blocking. Natalya Lukoyanova, Margaret S VanLoock, Albina Orlova, Vitold E Galkin, Kuan Wang, *Edward H Egelman* Published in Current biology : CB, March 2002 (see publication) Research themes: Type: Paper

2001

Molecular evolution: actin’s long lost relative found. *E H Egelman* Published in Current biology : CB, December 2001 (see publication) Research themes: Type: Paper, Corresponding author

Archaeal RadA protein binds DNA as both helical filaments and octameric rings. S Yang, X Yu, E M Seitz, S C Kowalczykowski, *E H Egelman* Published in Journal of molecular biology, December 2001 (see publication) Research themes: Type: Paper

Comparison of bacteriophage T4 UvsX and human Rad51 filaments suggests that RecA-like polymers may have evolved independently. S Yang, M S VanLoock, X Yu, *E H Egelman* Published in Journal of molecular biology, October 2001 (see publication) Research themes: Type: Paper

Electron microscopic studies of the translin octameric ring. M S VanLoock, X Yu, M Kasai, *E H Egelman* Published in Journal of structural biology, September 2001 (see publication) Research themes: Type: Paper

Probing the structure of F-actin: cross-links constrain atomic models and modify actin dynamics. A Orlova, V E Galkin, M S VanLoock, E Kim, A Shvetsov, E Reisler, *E H Egelman* Published in Journal of molecular biology, September 2001 (see publication) Research themes: Type: Paper

The primase active site is on the outside of the hexameric bacteriophage T7 gene 4 helicase-primase ring. M S VanLoock, Y J Chen, X Yu, S S Patel, *E H Egelman* Published in Journal of molecular biology, September 2001 (see publication) Research themes: Type: Paper

Actin allostery again? *E H Egelman* Published in Nature structural biology, August 2001 (see publication) Research themes: Type: Paper

Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA. X Yu, S A Jacobs, S C West, T Ogawa, *E H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, July 2001 (see publication) Research themes: Type: Paper

Does a stretched DNA structure dictate the helical geometry of RecA-like filaments? *E H Egelman* Published in Journal of molecular biology, June 2001 (see publication) Research themes: Type: Paper, Corresponding author

Actin depolymerizing factor stabilizes an existing state of F-actin and can change the tilt of F-actin subunits. V E Galkin, A Orlova, N Lukoyanova, W Wriggers, *E H Egelman* Published in The Journal of cell biology, April 2001 (see publication) Research themes: Type: Paper

Binding of dystrophin’s tandem calponin homology domain to F-actin is modulated by actin’s structure. A Orlova, I N Rybakova, E Prochniewicz, D D Thomas, J M Ervasti, *E H Egelman* Published in Biophysical journal, March 2001 (see publication) Research themes: Type: Paper

Bacterial conjugation: running rings around DNA. *E H Egelman* Published in Current biology : CB, March 2001 (see publication) Research themes: Type: Paper, Corresponding author

Structural biology. Pumping DNA. *E H Egelman* Published in Nature, February 2001 (see publication) Research themes: Type: Paper

2000

Two conformations of G-actin related to two conformations of F-actin. *E H Egelman*, A Orlova Published in Results and problems in cell differentiation, December 2000 (see publication) Research themes: Type: Paper

Three-dimensional reconstruction of transcription termination factor rho: orientation of the N-terminal domain and visualization of an RNA-binding site. X Yu, T Horiguchi, K Shigesada, *E H Egelman* Published in Journal of molecular biology, June 2000 (see publication) Research themes: Type: Paper

The human Rad52 protein exists as a heptameric ring. A Z Stasiak, E Larquet, A Stasiak, S Müller, A Engel, E Van Dyck, S C West, *E H Egelman* Published in Current biology : CB, April 2000 (see publication) Research themes: Type: Paper

F-actin retains a memory of angular order. A Orlova, *E H Egelman* Published in Biophysical journal, March 2000 (see publication) Research themes: Type: Paper

Visualization of two binding sites for the Escherichia coli UmuD’(2)C complex (DNA pol V) on RecA-ssDNA filaments. E G Frank, N Cheng, C C Do, M E Cerritelli, I Bruck, M F Goodman, *E H Egelman*, R Woodgate, A C Steven Published in Journal of molecular biology, March 2000 (see publication) Research themes: Type: Paper

A robust algorithm for the reconstruction of helical filaments using single-particle methods. *E H Egelman* Published in Ultramicroscopy, January 2000 (see publication) Research themes: Type: Paper, Corresponding author

1999

Human Dmc1 protein binds DNA as an octameric ring. S I Passy, X Yu, Z Li, C M Radding, J Y Masson, S C West, *E H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, September 1999 (see publication) Research themes: Type: Paper

Rings and filaments of beta protein from bacteriophage lambda suggest a superfamily of recombination proteins. S I Passy, X Yu, Z Li, C M Radding, *E H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, April 1999 (see publication) Research themes: Type: Paper

Bacterial helicases. *E H Egelman* Published in Journal of structural biology, March 1999 (see publication) Research themes: Type: Paper

Biochemical and electron microscopic image analysis of the hexameric E1 helicase. E T Fouts, X Yu, *E H Egelman*, M R Botchan Published in The Journal of biological chemistry, February 1999 (see publication) Research themes: Type: Paper

Intrastrand cross-linked actin between Gln-41 and Cys-374. III. Inhibition of motion and force generation with myosin. E Kim, E Bobkova, C J Miller, A Orlova, G Hegyi, *E H Egelman*, A Muhlrad, E Reisler Published in Biochemistry, January 1999 (see publication) Research themes: Type: Paper

A change in actin conformation associated with filament instability after Pi release. L D Belmont, A Orlova, D G Drubin, *E H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, January 1999 (see publication) Research themes: Type: Paper

1998

Identification of a defined epitope on the surface of the active RecA-DNA filament using a monoclonal antibody and three-dimensional reconstruction. X Yu, T Shibata, *E H Egelman* Published in Journal of molecular biology, November 1998 (see publication) Research themes: Type: Paper

Tubulin family: kinship of key proteins across phylogenetic domains. *E H Egelman* Published in Current biology : CB, May 1998 (see publication) Research themes: Type: Paper, Corresponding author

1997

New angles on actin dynamics. *E H Egelman* Published in Structure (London, England : 1993), October 1997 (see publication) Research themes: Type: Paper, Corresponding author

Only one F-actin model. *E H Egelman*, A Orlova, A McGough Published in Nature structural biology, September 1997 (see publication) Research themes: Type: Paper

Modulation of yeast F-actin structure by a mutation in the nucleotide-binding cleft. A Orlova, X Chen, P A Rubenstein, *E H Egelman* Published in Journal of molecular biology, August 1997 (see publication) Research themes: Type: Paper

Sickle cell anemia research and a recombinant DNA technique. A Stasiak, S C West, *E H Egelman* Published in Science (New York, N.Y.), July 1997 (see publication) Research themes: Type: Paper

Structure and subunit composition of the RuvAB-Holliday junction complex. X Yu, S C West, *E H Egelman* Published in Journal of molecular biology, February 1997 (see publication) Research themes: Type: Paper

Cooperative rigor binding of myosin to actin is a function of F-actin structure. A Orlova, *E H Egelman* Published in Journal of molecular biology, February 1997 (see publication) Research themes: Type: Paper

The RecA hexamer is a structural homologue of ring helicases. X Yu, *E H Egelman* Published in Nature structural biology, February 1997 (see publication) Research themes: Type: Paper

1996

DNA is bound within the central hole to one or two of the six subunits of the T7 DNA helicase. X Yu, M M Hingorani, S S Patel, *E H Egelman* Published in Nature structural biology, September 1996 (see publication) Research themes: Type: Paper

Homomorphous hexameric helicases: tales from the ring cycle. *E H Egelman* Published in Structure (London, England : 1993), July 1996 (see publication) Research themes: Type: Paper, Corresponding author

The hexameric E. coli DnaB helicase can exist in different Quaternary states. X Yu, M J Jezewska, W Bujalowski, *E H Egelman* Published in Journal of molecular biology, May 1996 (see publication) Research themes: Type: Paper

1995

Motile systems: Tubulin-based motility races ahead. *E H Egelman* Published in Current biology : CB, December 1995 (see publication) Research themes: Type: Paper

Structural polymorphism of the RecA protein from the thermophilic bacterium Thermus aquaticus. X Yu, E Angov, R D Camerini-Otero, *E H Egelman* Published in Biophysical journal, December 1995 (see publication) Research themes: Type: Paper

Allostery, cooperativity, and different structural states in F-actin. *E H Egelman*, A Orlova Published in Journal of structural biology, September 1995 (see publication) Research themes: Type: Paper

Bacteriophage T7 helicase/primase proteins form rings around single-stranded DNA that suggest a general structure for hexameric helicases. *E H Egelman*, X Yu, R Wild, M M Hingorani, S S Patel Published in Proceedings of the National Academy of Sciences of the United States of America, April 1995 (see publication) Research themes: Type: Paper

New insights into actin filament dynamics. *E H Egelman*, A Orlova Published in Current opinion in structural biology, April 1995 (see publication) Research themes: Type: Paper

Structural dynamics of F-actin: I. Changes in the C terminus. A Orlova, *E H Egelman* Published in Journal of molecular biology, February 1995 (see publication) Research themes: Type: Paper

At least one of the protofilaments in flagellar microtubules is not composed of tubulin. D Nojima, R W Linck, *E H Egelman* Published in Current biology : CB, February 1995 (see publication) Research themes: Type: Paper

1994

The Escherichia coli RuvB branch migration protein forms double hexameric rings around DNA. A Stasiak, I R Tsaneva, S C West, C J Benson, X Yu, *E H Egelman* Published in Proceedings of the National Academy of Sciences of the United States of America, August 1994 (see publication) Research themes: Type: Paper

The bacteriophage phi 29 head-tail connector shows 13-fold symmetry in both hexagonally packed arrays and as single particles. V Tsuprun, D Anderson, *E H Egelman* Published in Biophysical journal, June 1994 (see publication) Research themes: Type: Paper

Structure and function of RecA-DNA complexes. A Stasiak, *E H Egelman* Published in Experientia, March 1994 (see publication) Research themes: Type: Paper

Three-dimensional reconstruction of a co-complex of F-actin with antibody Fab fragments to actin’s NH2 terminus. A Orlova, X Yu, *E H Egelman* Published in Biophysical journal, February 1994 (see publication) Research themes: Type: Paper

The twist, writhe and overall shape of supercoiled DNA change during counterion-induced transition from a loosely to a tightly interwound superhelix. Possible implications for DNA structure in vivo. J Bednar, P Furrer, A Stasiak, J Dubochet, *E H Egelman*, A D Bates Published in Journal of molecular biology, January 1994 (see publication) Research themes: Type: Paper

Actin filament structure. The ghost of ribbons past. *E H Egelman* Published in Current biology : CB, January 1994 (see publication) Research themes: Type: Paper

1993

A conformational change in the actin subunit can change the flexibility of the actin filament. A Orlova, *E H Egelman* Published in Journal of molecular biology, July 1993 (see publication) Research themes: Type: Paper

DNA conformation induced by the bacteriophage T4 UvsX protein appears identical to the conformation induced by the Escherichia coli RecA protein. X Yu, *E H Egelman* Published in Journal of molecular biology, July 1993 (see publication) Research themes: Type: Paper

The LexA repressor binds within the deep helical groove of the activated RecA filament. X Yu, *E H Egelman* Published in Journal of molecular biology, May 1993 (see publication) Research themes: Type: Paper

Similarity of the yeast RAD51 filament to the bacterial RecA filament. T Ogawa, X Yu, A Shinohara, *E H Egelman* Published in Science (New York, N.Y.), March 1993 (see publication) Research themes: Type: Paper

RecA-like recombination proteins in eukaryotes: functions and structures of RAD51 genes. T Ogawa, A Shinohara, A Nabetani, T Ikeya, X Yu, *E H Egelman*, H Ogawa Published in Cold Spring Harbor symposia on quantitative biology, January 1993 (see publication) Research themes: Type: Paper

1992

Image analysis shows that variations in actin crossover spacings are random, not compensatory. *E H Egelman*, D J DeRosier Published in Biophysical journal, November 1992 (see publication) Research themes: Type: Paper

Structural basis for the destabilization of F-actin by phosphate release following ATP hydrolysis. A Orlova, *E H Egelman* Published in Journal of molecular biology, October 1992 (see publication) Research themes: Type: Paper

Structural data suggest that the active and inactive forms of the RecA filament are not simply interconvertible. X Yu, *E H Egelman* Published in Journal of molecular biology, September 1992 (see publication) Research themes: Type: Paper

Direct visualization of dynamics and co-operative conformational changes within RecA filaments that appear to be associated with the hydrolysis of adenosine 5’-O-(3-thiotriphosphate).